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	<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/api.php?action=feedcontributions&amp;feedformat=atom&amp;user=Admin</id>
	<title>Taiwan beetles - User contributions [en]</title>
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	<updated>2026-10-25T20:52:27Z</updated>
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	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=378</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=378"/>
		<updated>2026-09-23T05:15:06Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:flex; flex-wrap:wrap; align-items:flex-start;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_NSYSU.png|220x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_NMNS.jpg|220x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_Prague.png|180x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_Copenhagen.png|180x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_BiologyCentre.png|180x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
== Funding ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:flex; flex-wrap:wrap; align-items:flex-start;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_NSTC.png|220x80px|link=https://www.nstc.gov.tw/|alt=National Science and Technology Council]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Science and Technology Council (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:280px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_GACR.png|220x80px|link=https://gacr.cz/en/|alt=Czech Science Foundation]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Czech Science Foundation (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_NSTC.png&amp;diff=377</id>
		<title>File:Logo NSTC.png</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_NSTC.png&amp;diff=377"/>
		<updated>2026-09-23T05:13:12Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_GACR.png&amp;diff=375</id>
		<title>File:Logo GACR.png</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_GACR.png&amp;diff=375"/>
		<updated>2026-09-23T05:06:59Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=373</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=373"/>
		<updated>2026-09-23T04:56:24Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:flex; flex-wrap:wrap; align-items:flex-start;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_NSYSU.png|220x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_NMNS.jpg|220x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_Prague.png|180x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_Copenhagen.png|180x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_BiologyCentre.png|180x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
== Funding ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:flex; flex-wrap:wrap; align-items:flex-start;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_NSTC.png|220x80px|link=https://www.nstc.gov.tw/|alt=National Science and Technology Council]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Science and Technology Council (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_GACR.png|220x80px|link=https://gacr.cz/en/|alt=Czech Science Foundation]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Czech Science Foundation (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=372</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=372"/>
		<updated>2026-09-23T04:49:59Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:flex; flex-wrap:wrap; align-items:flex-start;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_NSYSU.png|220x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:220px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_NMNS.jpg|220x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_Prague.png|180x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_Copenhagen.png|180x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;width:180px; margin:0.5em 2em 0.5em 0; text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;height:90px; display:flex; align-items:flex-end; justify-content:center;&amp;quot;&amp;gt;[[File:Logo_BiologyCentre.png|180x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=371</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=371"/>
		<updated>2026-09-23T04:47:22Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:220px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NSYSU.png|220x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:220px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NMNS.jpg|220x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Prague.png|180x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Copenhagen.png|180x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_BiologyCentre.png|180x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=370</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=370"/>
		<updated>2026-09-23T04:46:55Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NSYSU.png|220x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NMNS.jpg|220x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Prague.png|180x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Copenhagen.png|180x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_BiologyCentre.png|180x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=369</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=369"/>
		<updated>2026-09-23T04:46:20Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NSYSU.png|180x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NMNS.jpg|180x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Prague.png|180x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Copenhagen.png|180x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_BiologyCentre.png|180x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_Copenhagen.png&amp;diff=368</id>
		<title>File:Logo Copenhagen.png</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_Copenhagen.png&amp;diff=368"/>
		<updated>2026-09-23T04:42:02Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=367</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=367"/>
		<updated>2026-09-23T04:40:03Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NSYSU.png|x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NMNS.jpg|x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Prague.png|x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Copenhagen.png|x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_BiologyCentre.png|x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=366</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=366"/>
		<updated>2026-09-23T04:39:33Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|400px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NSYSU.png|x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NMNS.jpg|x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Prague.png|x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Copenhagen.png|x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_BiologyCentre.png|x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_BiologyCentre.png&amp;diff=365</id>
		<title>File:Logo BiologyCentre.png</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_BiologyCentre.png&amp;diff=365"/>
		<updated>2026-09-23T04:38:59Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_Prague.png&amp;diff=364</id>
		<title>File:Logo Prague.png</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_Prague.png&amp;diff=364"/>
		<updated>2026-09-23T04:36:44Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=363</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=363"/>
		<updated>2026-09-23T04:34:54Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|400px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NSYSU.png|x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NMNS.jpg|x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Prague.png|x80px|link=https://www.nm.cz/en|alt=National Museum Prague]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Copenhagen.png|x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_BiologyCentre.png|x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=362</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=362"/>
		<updated>2026-09-23T04:32:37Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|400px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NSYSU.png|x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NMNS.jpg|x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Prague.png|x80px|link=|alt=National University Prague]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National University Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Copenhagen.png|x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_BiologyCentre.png|x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_NMNS.jpg&amp;diff=361</id>
		<title>File:Logo NMNS.jpg</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_NMNS.jpg&amp;diff=361"/>
		<updated>2026-09-23T04:30:44Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_NSYSU.png&amp;diff=360</id>
		<title>File:Logo NSYSU.png</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Logo_NSYSU.png&amp;diff=360"/>
		<updated>2026-09-23T04:28:29Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=359</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=359"/>
		<updated>2026-09-23T04:22:58Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|400px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
== Participating institutions ==&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center;&amp;quot;&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NSYSU.png|x80px|link=https://www.nsysu.edu.tw/|alt=National Sun Yat-sen University]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Sun Yat-sen University (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_NMNS.png|x80px|link=https://www.nmns.edu.tw/|alt=National Museum of Natural Science]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National Museum of Natural Science (Taiwan)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Prague.png|x80px|link=|alt=National University Prague]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;National University Prague (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_Copenhagen.png|x80px|link=https://www.ku.dk/|alt=University of Copenhagen]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;University of Copenhagen (Denmark)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;display:inline-block; width:180px; margin:0.5em 1em; vertical-align:top;&amp;quot;&amp;gt;&lt;br /&gt;
[[File:Logo_BiologyCentre.png|x80px|link=https://www.bc.cas.cz/|alt=Biology Centre CAS]]&amp;lt;br/&amp;gt;&lt;br /&gt;
&amp;lt;small&amp;gt;Biology Centre, Czech Academy of Sciences (Czech Republic)&amp;lt;/small&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=358</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=358"/>
		<updated>2026-09-23T04:21:16Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|400px|Simplified workflow of the project]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=357</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=357"/>
		<updated>2026-09-23T04:17:37Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|400px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Workflow.png&amp;diff=356</id>
		<title>File:Workflow.png</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Workflow.png&amp;diff=356"/>
		<updated>2026-09-23T04:16:44Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=355</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=355"/>
		<updated>2026-09-23T04:11:16Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|400px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=354</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=354"/>
		<updated>2026-09-23T04:10:45Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle morphospecies:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field (at least 4 litres of leaf litter per sample).&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; using the HotShot protocol (one specimen of each morphospecies in each sample)&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=352</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=352"/>
		<updated>2026-09-23T04:06:17Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
[[File:Workflow.png|thumb|right|350px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=351</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=351"/>
		<updated>2026-09-23T04:03:19Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=350</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=350"/>
		<updated>2026-09-23T04:01:49Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;border:2px solid #E02020; background:#FDECEA; color:#E02020; padding:0.6em 1em; margin:1em 0; font-size:105%; overflow:hidden;&amp;quot;&amp;gt;&lt;br /&gt;
⚠️ This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=349</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=349"/>
		<updated>2026-09-23T04:00:04Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;font-size:120%; font-weight:bold; color:#B22222; margin:1em 0;&amp;quot;&amp;gt;&lt;br /&gt;
This wiki was just started: lots of information will be added soon!&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=348</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=348"/>
		<updated>2026-09-23T03:53:18Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples by providing:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification, ideally done by taxonomic experts&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=347</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=347"/>
		<updated>2026-09-23T03:52:04Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;: we aim at collecting at least 300 samples across Taiwan, from lowlands to high mountains, from pristine forest to plantations and city forests.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Build up open-access DNA barcode library&#039;&#039;&#039; that will allow for a quick identification and make leaf litter beetles in Taiwan accessible for ecological or forestry studies.&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology, altitudinal distribution, types of forests inhabited, etc.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=346</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=346"/>
		<updated>2026-09-23T03:46:59Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:260px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=345</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=345"/>
		<updated>2026-09-23T03:46:44Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:340px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|- style=&amp;quot;border-top:1px solid #E3D6C4;&amp;quot;&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=344</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=344"/>
		<updated>2026-09-23T03:45:38Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:250px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=343</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=343"/>
		<updated>2026-09-23T03:43:30Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:340px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Often, they are very tiny, some of them can be very common. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
&lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Our project aims to accumulate the basic data about diversity and distribution of leaf litter beetles in Taiwan. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=342</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=342"/>
		<updated>2026-09-23T03:40:19Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:340px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Why forest leaf litter? ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects that are keeping the forest alive - they are decomposers, predators, fungivores or parasitoids. Most of these insects are very tiny, but some can be very common and numerous. The combination of tiny size, high diversity and high number of specimens makes them difficult to study - one sample of leaf litter often includes so many species and individuals that it may need weeks of work to get their basic identifications. Yet, without knowing who they are, we cannot study their biodiversity and ecology, and to understand whether and how are they involved in the life of the forest.  &lt;br /&gt;
Many of these tiny leaf litter insects are &#039;&#039;&#039;beetles&#039;&#039;&#039;: one sample of leaf litter in Taiwan may contain up to 900 individuals of beetles belonging to 100 species. Beetles are also diverse in their diets and life styles, making them a good proxy group to understand how forest floor invertebrate community works. And they are more popular than other groups, and hence more easy in finding students to work with them and researchers to help with their identifications.  &lt;br /&gt;
&lt;br /&gt;
Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=341</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=341"/>
		<updated>2026-09-23T03:28:50Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:340px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Welcome ==&lt;br /&gt;
&lt;br /&gt;
The forest floor is home to an enormous diversity of insects: decomposers, predators, fungivores and parasitoids. Many of them are &#039;&#039;&#039;beetles&#039;&#039;&#039;. Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=340</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=340"/>
		<updated>2026-09-23T03:28:07Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Welcome ==&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|250px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:340px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
The forest floor is home to an enormous diversity of insects: decomposers, predators, fungivores and parasitoids. Many of them are &#039;&#039;&#039;beetles&#039;&#039;&#039;. Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=339</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=339"/>
		<updated>2026-09-23T03:27:40Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Welcome ==&lt;br /&gt;
[[File:Sampled_localities.png|thumb|right|340px|Sampled localities across Taiwan (last update: September 2026)]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:340px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
The forest floor is home to an enormous diversity of insects: decomposers, predators, fungivores and parasitoids. Many of them are &#039;&#039;&#039;beetles&#039;&#039;&#039;. Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=338</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=338"/>
		<updated>2026-09-23T03:25:49Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Welcome ==&lt;br /&gt;
[[File:Sampled_localities_map.png|thumb|right|340px|Sampled localities across Taiwan]]&lt;br /&gt;
{| style=&amp;quot;float:right; clear:right; width:340px; margin:0 0 1em 1em; border:1px solid #C9B49A; background:#FBF8F3; border-collapse:collapse;&amp;quot;&lt;br /&gt;
|+ style=&amp;quot;background:#7B4A1E; color:#FFFFFF; padding:0.4em;&amp;quot; | &#039;&#039;&#039;Project in numbers&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Specimens sorted || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;39,730&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Samples sorted and sequenced || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;157&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Individuals with high-quality sequences || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;5,466&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Potential beetle species (OTUs) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;1,543&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| Papers published (2021–2026) || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;11&#039;&#039;&#039;&lt;br /&gt;
|-&lt;br /&gt;
| New species described / in progress || style=&amp;quot;text-align:right;&amp;quot; | &#039;&#039;&#039;45&#039;&#039;&#039;&lt;br /&gt;
|}&lt;br /&gt;
The forest floor is home to an enormous diversity of insects: decomposers, predators, fungivores and parasitoids. Many of them are &#039;&#039;&#039;beetles&#039;&#039;&#039;. Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Sampled_localities.png&amp;diff=337</id>
		<title>File:Sampled localities.png</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Sampled_localities.png&amp;diff=337"/>
		<updated>2026-09-23T03:23:52Z</updated>

		<summary type="html">&lt;p&gt;Admin: Map of samples localities (last update: September 2026)&lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;== Summary ==&lt;br /&gt;
Map of samples localities (last update: September 2026)&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=336</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=336"/>
		<updated>2026-09-23T03:13:24Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:250%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:170%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Welcome ==&lt;br /&gt;
The forest floor is home to an enormous diversity of insects: decomposers, predators, fungivores and parasitoids. Many of them are &#039;&#039;&#039;beetles&#039;&#039;&#039;. Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=MediaWiki:Common.css&amp;diff=335</id>
		<title>MediaWiki:Common.css</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=MediaWiki:Common.css&amp;diff=335"/>
		<updated>2026-09-23T03:10:34Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;/* ── Background ── */&lt;br /&gt;
body, #content {&lt;br /&gt;
    background-color: #f9f3ee;&lt;br /&gt;
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}&lt;br /&gt;
/* ── Top header bar ── */&lt;br /&gt;
#mw-head, .mw-header {&lt;br /&gt;
    background-color: #4a1e1e;&lt;br /&gt;
}&lt;br /&gt;
/* ── Sidebar ── */&lt;br /&gt;
#mw-panel {&lt;br /&gt;
    background-color: #f9f3ee;&lt;br /&gt;
}&lt;br /&gt;
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}&lt;br /&gt;
#mw-panel a {&lt;br /&gt;
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}&lt;br /&gt;
#mw-panel a:hover {&lt;br /&gt;
    color: #7a5c1e;&lt;br /&gt;
}&lt;br /&gt;
/* ── Page headings ── */&lt;br /&gt;
h1, h2, h3 {&lt;br /&gt;
    color: #4a1e1e;&lt;br /&gt;
}&lt;br /&gt;
h2 {&lt;br /&gt;
    border-bottom: 1px solid #7a5c1e;&lt;br /&gt;
}&lt;br /&gt;
/* ── Links ── */&lt;br /&gt;
a {&lt;br /&gt;
    color: #7a5c1e;&lt;br /&gt;
}&lt;br /&gt;
a:hover {&lt;br /&gt;
    color: #4a1e1e;&lt;br /&gt;
}&lt;br /&gt;
/* ── Tables ── */&lt;br /&gt;
.wikitable th {&lt;br /&gt;
    background-color: #4a1e1e;&lt;br /&gt;
    color: #f9f3ee;&lt;br /&gt;
}&lt;br /&gt;
.wikitable tr:nth-child(even) {&lt;br /&gt;
    background-color: #f0e8df;&lt;br /&gt;
}&lt;br /&gt;
/* ── Sidebar order ── */&lt;br /&gt;
#column-one {&lt;br /&gt;
    display: flex;&lt;br /&gt;
    flex-direction: column;&lt;br /&gt;
}&lt;br /&gt;
#p-search {&lt;br /&gt;
    order: 10;&lt;br /&gt;
}&lt;br /&gt;
#p-tb {&lt;br /&gt;
    order: 11;&lt;br /&gt;
}&lt;br /&gt;
#p-lang {&lt;br /&gt;
    order: 12;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
/* ── Sidebar order ── */&lt;br /&gt;
#p-search {&lt;br /&gt;
    display: none;&lt;br /&gt;
}&lt;br /&gt;
&lt;br /&gt;
body.page-Main_Page h1.firstHeading,&lt;br /&gt;
body.page-Main_Page #firstHeading {&lt;br /&gt;
    display: none;&lt;br /&gt;
}&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=334</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=334"/>
		<updated>2026-09-23T03:06:13Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:200%; font-weight:bold; margin:0.5em 0; color:#7B4A1E;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:left; font-size:115%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Welcome ==&lt;br /&gt;
The forest floor is home to an enormous diversity of insects: decomposers, predators, fungivores and parasitoids. Many of them are &#039;&#039;&#039;beetles&#039;&#039;&#039;. Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=333</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=333"/>
		<updated>2026-09-23T03:04:49Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:right; font-size:170%; font-weight:bold; margin:0.5em 0;&amp;quot;&amp;gt;&lt;br /&gt;
Taiwan Leaf litter Beetles Project&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:right; font-size:115%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
Exploring the hidden diversity of beetles in Taiwanese forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Welcome ==&lt;br /&gt;
The forest floor is home to an enormous diversity of insects: decomposers, predators, fungivores and parasitoids. Many of them are &#039;&#039;&#039;beetles&#039;&#039;&#039;. Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=332</id>
		<title>Main Page</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Main_Page&amp;diff=332"/>
		<updated>2026-09-23T02:54:04Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;__NOTOC__&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center; font-size:170%; font-weight:bold; margin:0.5em 0;&amp;quot;&amp;gt;&lt;br /&gt;
Uncovering insect diversity hidden in subtropical forests&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div style=&amp;quot;text-align:center; font-size:115%; margin-bottom:1.5em;&amp;quot;&amp;gt;&lt;br /&gt;
DNA-based assessment of leaf litter beetles in Taiwan&lt;br /&gt;
&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== Welcome ==&lt;br /&gt;
The forest floor is home to an enormous diversity of insects: decomposers, predators, fungivores and parasitoids. Many of them are &#039;&#039;&#039;beetles&#039;&#039;&#039;. Despite their high species diversity and ecological importance, almost nothing is known about them, and identifying them is very difficult or even impossible.&lt;br /&gt;
&lt;br /&gt;
Our project aims to change that. We sample leaf litter across Taiwan, sort out the beetles, and use &#039;&#039;&#039;DNA barcoding&#039;&#039;&#039; to find out which species live where, how they live, and how diverse and unique Taiwan&#039;s forest-floor fauna really is.&lt;br /&gt;
&lt;br /&gt;
== Project goals ==&lt;br /&gt;
* &#039;&#039;&#039;Sample the forest floor across Taiwan&#039;&#039;&#039;, from lowlands to high mountains.&lt;br /&gt;
* &#039;&#039;&#039;Catalogue all beetle species&#039;&#039;&#039; found in the samples:&lt;br /&gt;
** &#039;&#039;cox1&#039;&#039; DNA barcodes&lt;br /&gt;
** species identification&lt;br /&gt;
** distribution&lt;br /&gt;
* &#039;&#039;&#039;Collect basic biology data&#039;&#039;&#039;, such as larval morphology and ecology.&lt;br /&gt;
* &#039;&#039;&#039;Map diversity and endemism in Taiwan&#039;&#039;&#039; by combining the data of all species.&lt;br /&gt;
&lt;br /&gt;
== How we work ==&lt;br /&gt;
Our workflow goes from a bag of leaf litter to DNA barcodes for every beetle:&lt;br /&gt;
&lt;br /&gt;
# &#039;&#039;&#039;Collecting&#039;&#039;&#039; – sifting forest leaf litter in the field.&lt;br /&gt;
# &#039;&#039;&#039;Extracting insects&#039;&#039;&#039; – using Winkler extractors.&lt;br /&gt;
# &#039;&#039;&#039;Sorting&#039;&#039;&#039; – picking out all beetles from each sample.&lt;br /&gt;
# &#039;&#039;&#039;DNA extraction&#039;&#039;&#039; – using the HotShot protocol.&lt;br /&gt;
# &#039;&#039;&#039;PCR amplification&#039;&#039;&#039; of the &#039;&#039;cox1&#039;&#039; gene – two reactions per specimen (full 658 bp barcode and a shorter 407 bp fragment) to increase success rate.&lt;br /&gt;
# &#039;&#039;&#039;Sequencing&#039;&#039;&#039; – pooled PCR products are sequenced directly in our lab (up to 2,500 samples at once).&lt;br /&gt;
# &#039;&#039;&#039;Analysis&#039;&#039;&#039; – DNA barcodes are used to delimit species, match larvae with adults, and map where species occur.&lt;br /&gt;
&lt;br /&gt;
== Example: mysterious larvae with cases ==&lt;br /&gt;
Beetle larvae that live inside cases are common in leaf litter all over Taiwan, but for a long time nobody knew what they were.&lt;br /&gt;
&lt;br /&gt;
* &#039;&#039;&#039;Identification:&#039;&#039;&#039; by matching larval DNA with adults, we identified them as &#039;&#039;Stenochinus&#039;&#039; (family Tenebrionidae). Only 7 rare endemic species of this genus had been reported from Taiwan.&lt;br /&gt;
* &#039;&#039;&#039;Species number:&#039;&#039;&#039; species delimitation revealed &#039;&#039;&#039;11 potential species&#039;&#039;&#039;, one of which also occurs in Japan.&lt;br /&gt;
* &#039;&#039;&#039;Distribution:&#039;&#039;&#039; most species live at low and middle altitudes, and they are found not only in natural forests but also in secondary forests and plantations.&lt;br /&gt;
&lt;br /&gt;
This shows how DNA barcoding can take a group of insects from &amp;quot;completely unknown&amp;quot; to having basic biodiversity data.&lt;br /&gt;
&lt;br /&gt;
== Where the data leads ==&lt;br /&gt;
By combining the data from all species, we can map:&lt;br /&gt;
* &#039;&#039;&#039;species diversity&#039;&#039;&#039; across Taiwan,&lt;br /&gt;
* &#039;&#039;&#039;endemism&#039;&#039;&#039; (species found only in Taiwan or small parts of it),&lt;br /&gt;
* expected &#039;&#039;&#039;diversity change by 2060&#039;&#039;&#039;, using species distribution models.&lt;br /&gt;
&lt;br /&gt;
These maps can help identify areas with high richness and endemism, with practical uses such as conservation management.&lt;br /&gt;
&lt;br /&gt;
== Project in numbers ==&lt;br /&gt;
{| class=&amp;quot;wikitable&amp;quot; style=&amp;quot;text-align:left;&amp;quot;&lt;br /&gt;
|-&lt;br /&gt;
! Specimens sorted&lt;br /&gt;
| 39,730&lt;br /&gt;
|-&lt;br /&gt;
! Samples sorted and sequenced&lt;br /&gt;
| 157&lt;br /&gt;
|-&lt;br /&gt;
! Individuals with high-quality sequences&lt;br /&gt;
| 5,466&lt;br /&gt;
|-&lt;br /&gt;
! Potential beetle species (OTUs)&lt;br /&gt;
| 1,543&lt;br /&gt;
|-&lt;br /&gt;
! Papers published (2021–2026)&lt;br /&gt;
| 11&lt;br /&gt;
|-&lt;br /&gt;
! New species described / in progress&lt;br /&gt;
| 45&lt;br /&gt;
|}&lt;br /&gt;
&#039;&#039;Numbers as of September 2026. The project is still running – more samples will be collected and more data generated.&#039;&#039;&lt;br /&gt;
&lt;br /&gt;
== Get involved ==&lt;br /&gt;
Our preliminary identifications need proper taxonomic work, so &#039;&#039;&#039;we are looking for collaborators!&#039;&#039;&#039; If you work on the taxonomy of any beetle group occurring in Taiwan, please get in touch.&lt;br /&gt;
&lt;br /&gt;
* Contact: Martin Fikáček – mfikacek@gmail.com&lt;br /&gt;
* Project website: [https://taiwanbeetles.net/ taiwanbeetles.net]&lt;br /&gt;
&lt;br /&gt;
== Team ==&lt;br /&gt;
Martin Fikáček, Bin-Hong Ho, Fang-Shuo Hu, Chun-Fang Chang, Chih-Chi Chen, Yu-Chieh Hsu, Chen-Wen Lin, Guan Jie Phang, Albert Damaška, Enrico Ruzzier&lt;br /&gt;
&lt;br /&gt;
&#039;&#039;&#039;Institutions:&#039;&#039;&#039; National Sun Yat-sen University (Taiwan); National Museum of Natural Science (Taiwan); University of Copenhagen (Denmark); Biology Centre, Czech Academy of Sciences (Czech Republic); World Biodiversity Association (Italy)&lt;br /&gt;
&lt;br /&gt;
== Funding ==&lt;br /&gt;
The project is supported by the National Science and Technology Council of Taiwan (113-2621-B-110-005-MY3) and by the Czech Science Foundation (25-15506S).&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Trichophyinae&amp;diff=331</id>
		<title>Trichophyinae</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Trichophyinae&amp;diff=331"/>
		<updated>2026-09-07T15:41:45Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:Trichophya_habitus.jpg|thumb|right|400px|Habitus of Taiwanese &#039;&#039;Trichophya&#039;&#039;, showing the differences between &#039;&#039;T. thor&#039;&#039; and the remaining species.]]&lt;br /&gt;
Trichophyinae is a small staphylinid subfamily with only 21 described species, all classified in a single genus &#039;&#039;Trichophya&#039;&#039;. They can be easily recognized from other Taiwanese Staphylinidae by unusually thin antennae.&lt;br /&gt;
&lt;br /&gt;
Four species of the genus are known from Taiwan, all endemic for the island:&lt;br /&gt;
* &#039;&#039;T. keris&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. kurosawai&#039;&#039; Shibata, 2001&amp;lt;sup&amp;gt;[[#ref2|[2]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. maui&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. thor&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
All four species have been collected in leaf litter of montane forests at elevations of 1300–2200 m, and seem to be Palaearctic elements of the Taiwanese fauna.&lt;br /&gt;
&lt;br /&gt;
Morphologically, Taiwanese species form two groups: &#039;&#039;T. kurosawai&#039;&#039; + &#039;&#039;T. keris&#039;&#039; + &#039;&#039;T. maui&#039;&#039; have a broader body, relatively longer elytra, and head and pronotum with scale-like microsculpture. In contrast, &#039;&#039;T. thor&#039;&#039; is more elongated, has shorter elytra, and the head and pronotum are smooth among the punctures. All four species can be easily recognized by the male genitalia.&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;clear:both;&amp;quot;&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
[[File:Trichophya_male_genitalia.jpg|thumb|left|600px|Male genitalia of Taiwanese &#039;&#039;Trichophya&#039;&#039; species.]]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;clear:both;&amp;quot;&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== References ==&lt;br /&gt;
&amp;lt;div id=&amp;quot;ref1&amp;quot;&amp;gt;&amp;lt;sup&amp;gt;&amp;lt;span style=&amp;quot;color:brown&amp;quot;&amp;gt;[1]&amp;lt;/span&amp;gt;&amp;lt;/sup&amp;gt; Hu F.-S., Ho B.-H. &amp;amp; Fikáček M. (2026): Revision of the genus &#039;&#039;Trichophya&#039;&#039; from Taiwan, with description of three new species (Staphylinidae: Trichophyinae). &#039;&#039;Taiwania&#039;&#039; 71(4): 667–674. [https://doi.org/10.6165/tai.2026.71.667 DOI]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div id=&amp;quot;ref2&amp;quot;&amp;gt;&amp;lt;sup&amp;gt;&amp;lt;span style=&amp;quot;color:brown&amp;quot;&amp;gt;[2]&amp;lt;/span&amp;gt;&amp;lt;/sup&amp;gt; Shibata Y. (2001): A new species of the genus &#039;&#039;Trichophya&#039;&#039; (Coleoptera, Staphylinidae) from Taiwan. &#039;&#039;Elytra&#039;&#039; 29: 352–357.&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Trichophyinae&amp;diff=330</id>
		<title>Trichophyinae</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Trichophyinae&amp;diff=330"/>
		<updated>2026-09-07T15:40:24Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;[[File:Trichophya_habitus.jpg|thumb|right|400px|Habitus of Taiwanese &#039;&#039;Trichophya&#039;&#039;, showing the differences between &#039;&#039;T. thor&#039;&#039; and the remaining species.]]&lt;br /&gt;
Trichophyinae is a small staphylinid subfamily with only 21 described species, all classified in a single genus &#039;&#039;Trichophya&#039;&#039;. They can be easily recognized from other Taiwanese Staphylinidae by unusually thin antennae.&lt;br /&gt;
&lt;br /&gt;
Four species of the genus are known from Taiwan, all endemic for the island:&lt;br /&gt;
* &#039;&#039;T. keris&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. kurosawai&#039;&#039; Shibata, 2001&amp;lt;sup&amp;gt;[[#ref2|[2]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. maui&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. thor&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
All four species have been collected in leaf litter of montane forests at elevations of 1300–2200 m, and seem to be Palaearctic elements of the Taiwanese fauna.&lt;br /&gt;
&lt;br /&gt;
Morphologically, Taiwanese species form two groups: &#039;&#039;T. kurosawai&#039;&#039; + &#039;&#039;T. keris&#039;&#039; + &#039;&#039;T. maui&#039;&#039; have a broader body, relatively longer elytra, and head and pronotum with scale-like microsculpture. In contrast, &#039;&#039;T. thor&#039;&#039; is more elongated, has shorter elytra, and the head and pronotum are smooth among the punctures. All four species can be easily recognized by the male genitalia.&lt;br /&gt;
&lt;br /&gt;
[[File:Trichophya_male_genitalia.jpg|thumb|left|600px|Male genitalia of Taiwanese &#039;&#039;Trichophya&#039;&#039; species.]]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;clear:both;&amp;quot;&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== References ==&lt;br /&gt;
&amp;lt;div id=&amp;quot;ref1&amp;quot;&amp;gt;&amp;lt;sup&amp;gt;&amp;lt;span style=&amp;quot;color:brown&amp;quot;&amp;gt;[1]&amp;lt;/span&amp;gt;&amp;lt;/sup&amp;gt; Hu F.-S., Ho B.-H. &amp;amp; Fikáček M. (2026): Revision of the genus &#039;&#039;Trichophya&#039;&#039; from Taiwan, with description of three new species (Staphylinidae: Trichophyinae). &#039;&#039;Taiwania&#039;&#039; 71(4): 667–674. [https://doi.org/10.6165/tai.2026.71.667 DOI]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div id=&amp;quot;ref2&amp;quot;&amp;gt;&amp;lt;sup&amp;gt;&amp;lt;span style=&amp;quot;color:brown&amp;quot;&amp;gt;[2]&amp;lt;/span&amp;gt;&amp;lt;/sup&amp;gt; Shibata Y. (2001): A new species of the genus &#039;&#039;Trichophya&#039;&#039; (Coleoptera, Staphylinidae) from Taiwan. &#039;&#039;Elytra&#039;&#039; 29: 352–357.&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Trichophyinae&amp;diff=329</id>
		<title>Trichophyinae</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Trichophyinae&amp;diff=329"/>
		<updated>2026-09-07T15:39:06Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;Trichophyinae is a small staphylinid subfamily with only 21 described species, all classified in a single genus &#039;&#039;Trichophya&#039;&#039;. They can be easily recognized from other Taiwanese Staphylinidae by unusually thin antennae.&lt;br /&gt;
&lt;br /&gt;
[[File:Trichophya_habitus.jpg|thumb|right|400px|Habitus of Taiwanese &#039;&#039;Trichophya&#039;&#039;, showing the differences between &#039;&#039;T. thor&#039;&#039; and the remaining species.]]&lt;br /&gt;
&lt;br /&gt;
Four species of the genus are known from Taiwan, all endemic for the island:&lt;br /&gt;
* &#039;&#039;T. keris&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. kurosawai&#039;&#039; Shibata, 2001&amp;lt;sup&amp;gt;[[#ref2|[2]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. maui&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. thor&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
&lt;br /&gt;
All four species have been collected in leaf litter of montane forests at elevations of 1300–2200 m, and seem to be Palaearctic elements of the Taiwanese fauna.&lt;br /&gt;
&lt;br /&gt;
Morphologically, Taiwanese species form two groups: &#039;&#039;T. kurosawai&#039;&#039; + &#039;&#039;T. keris&#039;&#039; + &#039;&#039;T. maui&#039;&#039; have a broader body, relatively longer elytra, and head and pronotum with scale-like microsculpture. In contrast, &#039;&#039;T. thor&#039;&#039; is more elongated, has shorter elytra, and the head and pronotum are smooth among the punctures. All four species can be easily recognized by the male genitalia.&lt;br /&gt;
&lt;br /&gt;
[[File:Trichophya_male_genitalia.jpg|thumb|left|600px|Male genitalia of Taiwanese &#039;&#039;Trichophya&#039;&#039; species.]]&lt;br /&gt;
&lt;br /&gt;
&amp;lt;div style=&amp;quot;clear:both;&amp;quot;&amp;gt;&amp;lt;/div&amp;gt;&lt;br /&gt;
&lt;br /&gt;
== References ==&lt;br /&gt;
&amp;lt;div id=&amp;quot;ref1&amp;quot;&amp;gt;&amp;lt;sup&amp;gt;&amp;lt;span style=&amp;quot;color:brown&amp;quot;&amp;gt;[1]&amp;lt;/span&amp;gt;&amp;lt;/sup&amp;gt; Hu F.-S., Ho B.-H. &amp;amp; Fikáček M. (2026): Revision of the genus &#039;&#039;Trichophya&#039;&#039; from Taiwan, with description of three new species (Staphylinidae: Trichophyinae). &#039;&#039;Taiwania&#039;&#039; 71(4): 667–674. [https://doi.org/10.6165/tai.2026.71.667 DOI]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div id=&amp;quot;ref2&amp;quot;&amp;gt;&amp;lt;sup&amp;gt;&amp;lt;span style=&amp;quot;color:brown&amp;quot;&amp;gt;[2]&amp;lt;/span&amp;gt;&amp;lt;/sup&amp;gt; Shibata Y. (2001): A new species of the genus &#039;&#039;Trichophya&#039;&#039; (Coleoptera, Staphylinidae) from Taiwan. &#039;&#039;Elytra&#039;&#039; 29: 352–357.&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Trichophya_male_genitalia.jpg&amp;diff=328</id>
		<title>File:Trichophya male genitalia.jpg</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Trichophya_male_genitalia.jpg&amp;diff=328"/>
		<updated>2026-09-07T15:35:25Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Trichophya_habitus.jpg&amp;diff=327</id>
		<title>File:Trichophya habitus.jpg</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=File:Trichophya_habitus.jpg&amp;diff=327"/>
		<updated>2026-09-07T15:32:58Z</updated>

		<summary type="html">&lt;p&gt;Admin: &lt;/p&gt;
&lt;hr /&gt;
&lt;div&gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
	</entry>
	<entry>
		<id>https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Trichophyinae&amp;diff=326</id>
		<title>Trichophyinae</title>
		<link rel="alternate" type="text/html" href="https://taiwanbeetles.net/domains/taiwanbeetles.net/index.php?title=Trichophyinae&amp;diff=326"/>
		<updated>2026-09-07T15:32:09Z</updated>

		<summary type="html">&lt;p&gt;Admin: Created page with &amp;quot;Trichophyinae is a small staphylinid subfamily with only 21 described species, all classified in a single genus &amp;#039;&amp;#039;Trichophya&amp;#039;&amp;#039;. They can be easily recognized from other Taiwanese Staphylinidae by unusually thin antennae.  Habitus of Taiwanese &amp;#039;&amp;#039;Trichophya&amp;#039;&amp;#039;, showing the differences between &amp;#039;&amp;#039;T. thor&amp;#039;&amp;#039; and the remaining species.  Four species of the genus are known from Taiwan, all endemic for the island: * &amp;#039;&amp;#039;T. keris&amp;#039;&amp;#039; H...&amp;quot;&lt;/p&gt;
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&lt;div&gt;Trichophyinae is a small staphylinid subfamily with only 21 described species, all classified in a single genus &#039;&#039;Trichophya&#039;&#039;. They can be easily recognized from other Taiwanese Staphylinidae by unusually thin antennae.&lt;br /&gt;
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[[File:Trichophya_habitus.jpg|thumb|center|500px|Habitus of Taiwanese &#039;&#039;Trichophya&#039;&#039;, showing the differences between &#039;&#039;T. thor&#039;&#039; and the remaining species.]]&lt;br /&gt;
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Four species of the genus are known from Taiwan, all endemic for the island:&lt;br /&gt;
* &#039;&#039;T. keris&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. kurosawai&#039;&#039; Shibata, 2001&amp;lt;sup&amp;gt;[[#ref2|[2]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. maui&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
* &#039;&#039;T. thor&#039;&#039; Hu, Ho &amp;amp; Fikáček, 2026&amp;lt;sup&amp;gt;[[#ref1|[1]]]&amp;lt;/sup&amp;gt;&lt;br /&gt;
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All four species have been collected in leaf litter of montane forests at elevations of 1300–2200 m, and seem to be Palaearctic elements of the Taiwanese fauna.&lt;br /&gt;
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Morphologically, Taiwanese species form two groups: &#039;&#039;T. kurosawai&#039;&#039; + &#039;&#039;T. keris&#039;&#039; + &#039;&#039;T. maui&#039;&#039; have a broader body, relatively longer elytra, and head and pronotum with scale-like microsculpture. In contrast, &#039;&#039;T. thor&#039;&#039; is more elongated, has shorter elytra, and the head and pronotum are smooth among the punctures. All four species can be easily recognized by the male genitalia.&lt;br /&gt;
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[[File:Trichophya_male_genitalia.jpg|thumb|center|500px|Male genitalia of Taiwanese &#039;&#039;Trichophya&#039;&#039; species.]]&lt;br /&gt;
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== References ==&lt;br /&gt;
&amp;lt;div id=&amp;quot;ref1&amp;quot;&amp;gt;&amp;lt;sup&amp;gt;&amp;lt;span style=&amp;quot;color:brown&amp;quot;&amp;gt;[1]&amp;lt;/span&amp;gt;&amp;lt;/sup&amp;gt; Hu F.-S., Ho B.-H. &amp;amp; Fikáček M. (2026): Revision of the genus &#039;&#039;Trichophya&#039;&#039; from Taiwan, with description of three new species (Staphylinidae: Trichophyinae). &#039;&#039;Taiwania&#039;&#039; 71(4): 667–674. [https://doi.org/10.6165/tai.2026.71.667 DOI]&amp;lt;/div&amp;gt;&lt;br /&gt;
&amp;lt;div id=&amp;quot;ref2&amp;quot;&amp;gt;&amp;lt;sup&amp;gt;&amp;lt;span style=&amp;quot;color:brown&amp;quot;&amp;gt;[2]&amp;lt;/span&amp;gt;&amp;lt;/sup&amp;gt; Shibata Y. (2001): A new species of the genus &#039;&#039;Trichophya&#039;&#039; (Coleoptera, Staphylinidae) from Taiwan. &#039;&#039;Elytra&#039;&#039; 29: 352–357.&amp;lt;/div&amp;gt;&lt;/div&gt;</summary>
		<author><name>Admin</name></author>
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