Main public logs
From Taiwan beetles
Jump to navigationJump to search
Combined display of all available logs of Taiwan beetles. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 02:44, 6 May 2026 Admin talk contribs created page Methods/DNA barcoding (Created page with " = DNA EXTRACTION USING HOTSHOT = == MIXING NEW BUFFER == === Preparation === '''Alkaline Buffer''' (= 25 mM NaOH + 0.2 mM disodium EDTA) # Premix 10M NaOH: #* 70 ml H₂O + 40g NaOH and adjust to 100 ml #* ''or'' #* 17.5 ml H₂O + 10g NaOH and adjust to 25 ml # To 25 ml of H₂O add: #* 62.5 μl of 10M NaOH #* 10 μl of 0.5 M disodium EDTA ---- '''Neutralizing Buffer''' (40 mM trisHCl) Mix the following: * 24 ml of H₂O * 1 ml of 1M trisHCl ---- === Extracti...")
