Scripts/How to use Demultiplexing script

You can download the script here.

Example file with data needed to prepare demultiplex file: Data_for_demultiplex_EXAMPLE.xlsx

Needed files

List of plates Overview of sequencing plates with their names, amplicon lengths, and assigned forward primers.	Plate maps Layout of samples across plates, showing which sample code occupies each well position (A1–H12).
Reverse primer map Map of reverse primers (HCO_13 series) assigned to each well position across the plate.	List of primers Full primer list including tag sequences, basic primer sequences, and the combined tagged primer sequences used for demultiplexing.